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Typing of sheep clinical isolates and identification of enterotoxigenic Clostridium perfringens strains by classical methods and by polymerase chain reaction (PCR) 1 1 Work is currently under way (involving the French Central Veterinary Research Laboratory and the Institut Pasteur in Paris) to validate a diagnostic kit for the typing of C. perfringens and for detection of the enterotoxin. Specific primers have been chosen based on the published genome sequences for α-toxin [1–3], β-toxin [4], ϵ-toxin [5] and enterotoxin [6]. |
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