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  Breast carcinoma, intratumour heterogeneity and histological grading, using geostatistics
Titel: Breast carcinoma, intratumour heterogeneity and histological grading, using geostatistics
Auteur: VĂ©nus Sharifi-Salamatian
Anne de Roquancourt
Jean Paul Rigaut
Verschenen in: Analytical cellular pathology
Paginering: Jaargang 20 (2001) nr. 2-3 pagina's 83-91
Jaar: 2001-04-01
Inhoud: Tumour progression is currently believed to result from genetic instability. Chromosomal patterns specific of a type of cancer are frequent even though phenotypic spatial heterogeneity is omnipresent. The latter is the usual cause of histological grading imprecision, a well documented problem, without any fully satisfactory solution up to now. The present article addresses this problem in breast carcinoma. The assessment of a genetic marker for human tumours requires quantifiable measures of intratumoral heterogeneity. If any invariance paradigm representing a stochastic or geostatistic function could be discovered, this might help in solving the grading problem. A novel methodological approach using geostatistics to measure heterogeneity is used. Twenty tumours from the three usual (Scarff-Bloom and Richardson) grades were obtained and paraffin sections stained by MIB-1 (Ki-67) and peroxidase staining. Whole two-dimensional sections were sampled. Morphometric grids of variable sizes allowed a simple and fast recording of positions of epithelial nuclei, marked or not by MIB-1. The geostatistical method is based here upon the asymptotic behaviour of dispersion variance. Measure of asymptotic exponent of dispersion variance shows an increase from grade 1 to grade 3. Preliminary results are encouraging: grades 1 and 3 on one hand and 2 and 3 on the other hand are totally separated. The final proof of an improved grading using this measure will of course require a confrontation with the results of survival studies.
Uitgever: IOS Press
Bronbestand: Elektronische Wetenschappelijke Tijdschriften

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