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Details for article 5 of 14 found articles
| Title: |
Chromatin texture from hematoxylin stained thyroid lesions |
| Author: |
Olga Ferrer-Roca José A. Pérez Gómez Maritza Estévez |
| Appeared in: |
Analytical cellular pathology |
| Paging: | Volume 17 (2001) nr. 4 pages 209-217 |
| Year: |
2001-04-01 |
| Contents: |
Quantitative aspects of cytology and histology should be considered in diagnostic standardisation processes. The present paper summarises the cytological differences detected in 75 thyroid lesions using a computerized textural analysis. Cells stained with progressive hematoxylin and taken from paraffin blocks were overlaid with the extracted texture. This technique was based on the lineal detection of a grey level gradient of the common logarithm of the integrated optical density (IOD) of each nucleus. Diffuse and nodular goiters (36 cases) were demonstrated to be composed of small cells containing high density texture that, on microscopical visual inspection, gave a salt and pepper appearance. The adenomatous goiters (2 cases) and adenomas (26 cases) were composed of low texture cells with a visual blurry or smudgy chromatin, while the atypical adenomas with capsular invasion (4 cases) were characterised by a woodworm nuclear appearance that produced the highest texture of the series. Finally, encapsulated folliculo-papillary carcinomas (3 cases) were composed of large clear nuclei with high IOD, low texture, and scattered lines that resulted in an empty grape skin aspect. Our findings seam to confirm the suitability of computerized textural techniques that aid in recognizing cell microscopic features objectively. The one used in the present work, based on a mathematical function of the DNA content of each individual nucleus (IOD), fulfills all microscopy detection criteria. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 5 of 14 found articles
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