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Details for article 3 of 4 found articles
| Title: |
p53 mutations and DNA ploidy in colorectal adenocarcinomas |
| Author: |
Paola Campomenosi Paola Assereto Massimo Bogliolo Gilberto Fronza Angelo Abbondandolo Antonella Capasso Patrizia F. Bellomo Roberto Monaco Anna Rapallo Andrea Sciutto Roberto Orecchia Elio Geido Walter Giaretti |
| Appeared in: |
Analytical cellular pathology |
| Paging: | Volume 17 (2001) nr. 1 pages 1-12 |
| Year: |
2001-04-01 |
| Contents: |
The p53 tumour suppressor gene has an important role in the the maintenance of genome stability and its mutational inactivation may be at the origin of aneuploidy in cancer cells. The aim of this study was to determine whether p53 mutations were associated to DNA aneuploidy, as assessed by flow cytometry, in colorectal adenocarcinomas. Analysis of p53 mutations spectrum of the sorted nuclei was done by Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing. Overall, we studied 20 adenocarcinomas, the corresponding control mucosa, and 7 lymph node metastases. Five tumours (25%) were DNA diploid, while 15 tumours (75%) were composed of DNA aneuploid and diploid subpopulations. DNA diploid control mucosa and adenocarcinomas showed no p53 mutations, while 60% of the tumours with DNA aneuploidy had p53 mutations. Therefore, p53 mutations occurred significantly more often in DNA aneuploid than in DNA diploid tumours (p<0.04, Fisher's exact test). Incidences of DNA aneuploidy and p53 mutations in lymph node metastases were 60 and 86%, respectively. In all tumours showing a p53 mutation, the wild-type allele was not or only bearly visible in DNA aneuploid cells suggesting that, in such cells, aneuploidy is accompanied by complete p53 functional inactivation. The present observations suggest that p53 mutations may have a role in the origin of aneuploidy at late stages of colorectal carcinogenesis. |
| Publisher: |
IOS Press |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 3 of 4 found articles
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