Digital Library
Close Browse articles from a journal
 
<< previous    next >>
     Journal description
       All volumes of the corresponding journal
         All issues of the corresponding volume
           All articles of the corresponding issues
                                       Details for article 7 of 10 found articles
 
 
  Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice
 
 
Title: Quantitative comparison of immunohistochemical staining intensity in tissues fixed in formalin and Histochoice
Author: D. Geoffrey Vince
Abdelghani Tbakhi
Ajeetkumar Gaddipati
Robert M. Cothren
J. Fredrick Cornhill
Raymond R. Tubbs
Appeared in: Analytical cellular pathology
Paging: Volume 15 (2001) nr. 2 pages 119-129
Year: 2001-04-01
Contents: Formaldehyde fixatives have traditionally been used to preserve tissues as they impart excellent morphological preservation. Formaldehyde fixes tissue by cross linking, a process which can reduce the antigenicity of tissue and weakens the intensity of immunohistochemical stains. Preliminary studies have shown that Histochoice tissue fixative offers equal or greater staining intensity than neutral buffered formalin (NBF). This study compares these fixatives quantitatively and presents the results in unambiguous statistical terms. Tissue samples were collected, bisected, and fixed in NBF or Histochoice. The sections were stained with a total of 21 antibodies, and color images were collected. The hue, saturation, and value were determined for each positive pixel and an ANOVA performed. Small differences in hue were noted in 8 of 21 cases. Histochoice-fixed tissue gave a greater mean saturation than NBF with 57.1% of the antibodies tested. No significant difference in the saturation was detected in 28.6% of the cases; NBF gave higher mean saturation levels with only 14.3% of the antibodies. Histochoice-fixed tissue was found to give lower values in 66.7% of cases than those prepared with NBF, indicating darker staining. These results show that Histochoice produces staining intensity that is comparable, and in many cases superior, to formalin.
Publisher: IOS Press
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

                             Details for article 7 of 10 found articles
 
<< previous    next >>
 
 Koninklijke Bibliotheek - National Library of the Netherlands