Caenorhabditis elegans ZC376.5 encodes a tRNA(m2 2G26)dimethyltransferase in which 246arginine is important for the enzyme activity 1 Abbreviations: In this paper we have followed the two nonconsistent rules for nomenclature in the yeast S. cerevisiae (Sc) and the worm C. elegans (Ce). The wild type genes are denoted Sc TRM1 and Ce Trm1, respectively. The mutant genes Sc trm1 and Ce Trm1(1), Ce Trm1(2), etc. or, if the effect of the mutation is known, Ce Trm1(Aa position Aa). The protein products are denoted Sc Trm1p and CeTRM1, and the recombinant gene product made in bacterial cells rScTrm1p and rCeTrm1p. HPLC, high-performance liquid chromatography; m2G, N2-methylguanosine; mm2 2G, N2, N2-dimethylguaosine; RT-PCR, reverse-transcription-polymerase chain reaction; nt, nucleotide. 1
Titel:
Caenorhabditis elegans ZC376.5 encodes a tRNA(m2 2G26)dimethyltransferase in which 246arginine is important for the enzyme activity 1 Abbreviations: In this paper we have followed the two nonconsistent rules for nomenclature in the yeast S. cerevisiae (Sc) and the worm C. elegans (Ce). The wild type genes are denoted Sc TRM1 and Ce Trm1, respectively. The mutant genes Sc trm1 and Ce Trm1(1), Ce Trm1(2), etc. or, if the effect of the mutation is known, Ce Trm1(Aa position Aa). The protein products are denoted Sc Trm1p and CeTRM1, and the recombinant gene product made in bacterial cells rScTrm1p and rCeTrm1p. HPLC, high-performance liquid chromatography; m2G, N2-methylguanosine; mm2 2G, N2, N2-dimethylguaosine; RT-PCR, reverse-transcription-polymerase chain reaction; nt, nucleotide. 1