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| Titel: |
Viscosity measurements of DNA solutions with and without condensing agents |
| Auteur: |
Laesecke, Arno Burger, Jessica L. |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 51 (2014) nr. 1 pagina's 15-28 |
| Jaar: |
2014-03-04 |
| Inhoud: |
Aqueous solutions calf thymus DNA at three concentrations were studied by viscosity measurements at ambient pressure. A gravitational capillary viscometer and a rotating cylinder viscometer were employed. Three polycations were tested as viscosity-reducing agents with measurements from 25°C to 50°C. The most significant viscosity reduction was achieved with spermine in a solution of DNA at 2 mg/ml concentration. Measurements of DNA solutions without agents from 10°C to 90°C revealed concentration-dependent viscosity maxima in the range from 50°C to 65°C. The associated increases in viscosity were as high as 400%. A two-liquids model for solutions of double- and single-stranded DNA was developed that closely represented the experimental data. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 6 gevonden artikelen
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