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| Titel: |
Effect of lanthanum on red blood cell deformability |
| Auteur: |
Alexy, Tamas Nemeth, Norbert Wenby, Rosalinda B. Bauersachs, Rupert M. Baskurt, Oguz K. Meiselman, Herbert J. |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 44 (2008) nr. 5-6 pagina's 361-373 |
| Jaar: |
2008-04-07 |
| Inhoud: |
Prior reports describing the effects of lanthanum (La3+) on red blood cells (RBC) have focused on the effects of this lanthanide on cell fusion or on membrane characteristics (e.g., ion movement across membrane, membrane protein aggregation); the present study explores its rheological and biophysical effects. Normal human RBC were exposed to La3+ levels up to 200 μM then tested for: (1) cellular deformability using a laser-based ektacytometer and an optical-based rheoscope; (2) membrane viscoelastic behavior via micropipettes; (3) surface charge via micro electrophoresis. La3+ concentrations of 12.5 to 200 μM caused dose-dependent decreases of deformability that were greatest at low stresses: these rheological changes were completely reversible upon removing La3+ from the media either by washing with La3+-free buffer or by suspending La3+-exposed cells in La3+-free media (i.e., viscous dextran solution). Both membrane shear elastic modulus and membrane surface viscosity were increased by 25–30% at 100 or 200 μM. As expected, La3+ decreased RBC electrophoretic mobility (EPM), with EPM inversely but not linearly associated with deformability; changes of EPM were also completely reversible. These results thus indicate novel aspects of RBC cellular and membrane rheological behavior yet raise questions regarding specific mechanisms responsible for La3+-induced alterations. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 9 gevonden artikelen
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