|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 13 van 43 gevonden artikelen
| Titel: |
Effect of Peroxisome Proliferator Activated Receptor (PPAR)γ agonists on prostaglandins cascade in joint cells |
| Auteur: |
Moulin, David Poleni, Paul-Emile Kirchmeyer, Mélanie Sebillaud, Sylvie Koufany, Meriem Netter, Patrick Terlain, Bernard Bianchi, Arnaud Jouzeau, Jean-Yves |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 43 (2006) nr. 3-4 pagina's 561-575 |
| Jaar: |
2006-09-07 |
| Inhoud: |
In response to inflammatory cytokines, chondrocytes and synovial fibroblasts produce high amounts of prostaglandins (PG) which self-perpetuate locally the inflammatory reaction. Prostaglandins act primarily through membrane receptors coupled to G proteins but also bind to nuclear Peroxisome Proliferator-Activated Receptors (PPARs). Amongst fatty acids, the cyclopentenone metabolite of PGD2, 15-deoxy-$\Delta\tsup{12,14}$PGJ2 (15d-PGJ2), was shown to be a potent ligand of the PPARγ isotype prone to inhibit the production of inflammatory mediators. As the stimulated synthesis of PGE2 originates from the preferential coupling of inducible enzymes, cyclooxygenase-2 (COX-2) and membrane PGE synthase-1 (mPGES-1), we investigated the potency of 15d-PGJ2 to regulate prostaglandins synthesis in rat chondrocytes stimulated with interleukin-1β (IL-1β). We demonstrated that 15d-PGJ2, but not the high-affinity PPARγ ligand rosiglitazone, decreased almost completely PGE2 synthesis and mPGES-1 expression. The inhibitory potency of 15d-PGJ2 was unaffected by changes in PPARγ expression and resulted from inhibition of NF-κB nuclear binding and IκBα sparing, secondary to reduced phosphorylation of IKKβ. Consistently with 15d-PGJ2 being a putative endogenous regulator of the inflammatory reaction if synthesized in sufficient amounts, the present data confirm the variable PPARγ-dependency of its effects in joint cells while underlining possible species and cell types specificities. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 13 van 43 gevonden artikelen
| << vorige | volgende >> |