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| Titel: |
Quantitative analysis of the effects of hyaluronan and aggrecan concentration and hyaluronan size on the elasticity of hyaluronan-aggrecan solutions |
| Auteur: |
M. Nishimura M. Kawata W. Yan A. Okamato H. Nishimura Y. Ozaki T. Hamada Y. Kato |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 41 (2004) nr. 5 pagina's 629-639 |
| Jaar: |
2004-10-11 |
| Inhoud: |
We determined elasticity (G′) and viscosity (G′) of various aggrecan-hyaluronan solutions using a controlled-stress rheometer with high (10 Hz) to low (0.1 Hz) frequencies. Aggrecan solution (50 mg/ml) alone showed little elasticity at any frequency, but the addition of 3300 kDa hyaluronan at 0.001-0.1 mg/ml markedly increased the elasticity, but not the viscosity, at all frequencies. Increasing hyaluronan concentration at >0.1 mg/ml did not further increase the elasticity of the aggrecan solution, and the elasticity of the aggrecan-hyaluronan complex solution reached a plateau at a 500 :1 (w/w) ratio. In studies with increasing concentrations of aggrecan and a constant concentration (0.5 mg/ml) of 3300 kDa hyaluronan, aggrecan induced elasticity only at >20 mg/ml, indicating the presence of a critical concentration for elasticity. In the presence of 50 mg/ml aggrecan, 1000 kDa hyaluronan had far less effect on the elasticity of the aggrecan solution than did 3300 kDa hyaluronan. These findings suggest that only ∼50% reduction in aggrecan concentration (<20 mg/ml), or reduced hyaluronan size (<1000 kDa) - compared with their physiological levels in young cartilage - can abolish the elastic network of the aggrecan-hyaluronan complex. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 3 van 5 gevonden artikelen
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