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| Titel: |
Effect of steady and oscillatory shear stress on F-actin content and distribution in neutrophils |
| Auteur: |
Huai-Qing Chen Wei Tian Yun-Shuang Chen Liang Li Jeffrey Raum K.-L. Paul Sung |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 41 (2004) nr. 5 pagina's 655-664 |
| Jaar: |
2004-10-11 |
| Inhoud: |
We investigated neutrophil activation, specifically F-actin content and distribution, in situations mimicking the in vivo environment using steady and oscillatory shear. Under low steady shear (<150 s−1) F-actin levels were decreased for both treated (n-formyl-L-methioryl-L-leucyl-L-phenylalanine (fMLP)) and untreated neutrophils. The F-actin content increased with a change to higher steady shear levels. Neutrophils show the same behavior of decreased F-actin content for oscillatory shear (26.7 s−1) as they did for steady shear. In both situations, the low shear levels caused a decrease in F-actin content. However, as the magnitude of the shear rate increased, cells showed a reversal to increasing F-actin content. Shear caused a decrease in F-actin in the cell cortex for both control and fMLP treated cells. Ctyochalasin B (CB), a common F-actin assembly blocker, significantly decreased F-actin content. The results indicate that neutrophils regulate their actin network based on the level and type of shear stress they encounter in the bloodstream. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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