|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 6 van 57 gevonden artikelen
| Titel: |
Caveolin-1 redistribution in human endothelial cells induced by laminar flow and cytokine |
| Auteur: |
Sun, R.J. Muller, S. Zhuang, F.Y. Stoltz, J.F. Wang, X. |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 40 (2002) nr. 1-3 pagina's 31-39 |
| Jaar: |
2002-10-28 |
| Inhoud: |
Caveolin-1 is a principal component of caveolae and is involved in signaling transduction in a number of cells. A hypothesis was proposed in this work that mechanical forces due to flow induce caveolin-1 translocation. So the changes of caveolin-1 expression and distribution in cultured endothelial cells (HUVECs) exposed to a steady laminar flow were studied. For comparing with the influence of cytokine, caveolin-1 in the cells stimulated by TNF-α was also investigated. Indirect immunofluorescence and double fluorescence labeling showed that in control cells, caveolin-1 was primarily localized on the cell surface, which corresponded to the peripheral distribution of F-actin, and presented some local concentrations. In the cells exposed to a laminar flow (1.0 Pa), caveolin-1 distribution showed a time-dependent variation. After 24 h of shear, the local concentration of caveolin-1 was found, in the most cells, at upstream side of cell body. Also more caveolin-1 molecules were observed in the cells. In contrast, TNF-α induced a decrease of caveolin-1 in cells. The redistribution of caveolin-1 seems to be correlated to F-actin organization. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 57 gevonden artikelen
| << vorige | volgende >> |