|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 18 van 31 gevonden artikelen
| Titel: |
Nitric oxide (NO) and cartilage metabolism: NO effects are modulated by superoxide in response to IL-1 |
| Auteur: |
Jouzeau, Jean-Yves Pacquelet, Sandrine Boileau, Christelle Nedelec, Emmanuelle Presle, Nathalie Netter, Patrick Terlain, Bernard |
| Verschenen in: |
Biorheology |
| Paginering: | Jaargang 39 (2002) nr. 1-2 pagina's 201-214 |
| Jaar: |
2002-07-11 |
| Inhoud: |
Nitric oxide (NO) is thought to mediate most effects of interleukin-1 (IL-1) on cartilage. In vitro evidence includes the decreased synthesis of extracellular matrix components, the abnormal cell renewal, the decreased production of IL-1 receptor antagonist, the induction of apoptosis and the enhanced sensitivity of chondrocytes to oxidative stress. Studies in NOS2-/- mice or administration of NO synthase inhibitors in animal models of joint disorders have confirmed its potent pathophysiological role in cartilage. Using L-NMMA (1 mM), as a NO synthase inhibitor, and CuDips (10 µM), as a SOD mimetic, we provide evidence that the inhibitory potency of IL-1β on proteoglycan synthesis and its stimulating effect on COX-2 activity depend both on NO and O-.2 production. Peroxynitrite formation is further demonstrated by the occurrence of 3-nitrotyrosines in chondrocytes stimulated in vitro with 2.5 ng/ml IL-1 and in femoral condyles of rats injected locally with 1 µg IL-1. Preliminary data suggest that such contribution of reactive oxygen species is not shared in common by IL-17, another NO-producing cytokine. We conclude that superoxide is a key modulator of NO-mediated effects in chondrocyte stimulated with IL-1 and that a combined therapy with NO synthase inhibitors and antioxidants may be promising for a full cartilage protection. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 18 van 31 gevonden artikelen
| << vorige | volgende >> |