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| Titel: |
Survival rate of preantral follicles derived from vitrified neonate mouse ovarian tissue by cryotop and conventional methods |
| Auteur: |
Eimani, H. Mamzoji, S.S. Soleimani, M.M. Abnosi, M.H. Valojerdi, M. Rezazadeh Yazdi, P. Eftekhari Shahverdi, A. Guorabi, H. |
| Verschenen in: |
BioFactors |
| Paginering: | Jaargang 31 (2008) nr. 2 pagina's 117-126 |
| Jaar: |
2008-09-12 |
| Inhoud: |
The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop and conventional methods. The ovaries of 14-day-old mice were separated and divided into four groups as following: Cryotop group, vitrified by Cryotop; CV (Conventional; CV) group, vitrified by conventional straw; toxicity test group and control group. After warming the vitrified ovaries, isolated preantral follicles from four groups were cultured for 4 days to compare survival rate and follicular growth between above-mentioned groups. Survival rate (97.3%) in toxicity test group alike the control group (98.7%) were significantly higher (P < 0.05) than the Cryotop (92.7%) and CV (47.7%) groups. Increase in follicle diameters after 4 days in Cryotop and CV groups was significantly lower (P < 0.05) than the control and toxicity test groups, but growth and survival rate of follicles in Cryotop group was significantly higher (P < 0.05) than the CV group. These results demonstrated that ovarian tissue vitrification by Cryotop highly preserves the viability rate of preantral follicles. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 7 van 7 gevonden artikelen
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