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| Titel: |
The Role of Sucrose in the Development of Oral Biofilm in a Simulated Mouth System |
| Auteur: |
Wan Nordini Hasnor Wan Ismail Fathilah Abdul Razak Zubaidah Hj Abdul Rahim |
| Verschenen in: |
OnLine journal of biological sciences |
| Paginering: | Jaargang 6 (2006) nr. 2 pagina's 62-66 |
| Jaar: |
2006 |
| Inhoud: |
The development of oral biofilm consisting of early settlers and late settlers in the presence and absence of sucrose using a simulated mouth model was investigated. The experiments were carried out by growing the early settlers’ biofilm consisting of Strep. mitis and Strep. sanguinis and late settlers’ biofilm of Strep. mutans in the simulated mouth system. Experiments using three different nutrient conditions (A, B and C) were carried out. In A, sterile saliva was used as the nutrient source in which the early settlers were suspended and pumped into the simulated mouth system and allowed togrow for 24 hrs. In B, 1% of BHI broth was added to the sterile saliva three times a day at 6 hrs interval to demonstrate the effect of glucose on the development of the biofilms. In C, the 1% BHI wassupplemented with 1% sucrose in order to show the effect of glucose in the presence of sucrose on the development of the biofilm. The experiments were subsequently repeated with the late settlers. In allthe experiments, A serves as the control where the nutrient source is minimal. The data collected were analyzed using Student t-test. From the results obtained, it was observed that both glucose and sucroseenhanced the development of early and late settlers’ biofilms. The growth of late settlers’ biofilm however, was found to be significantly higher in the presence of sucrose (93x106 CFU mL¯1) compared to glucose (66x106 CFU mL¯1) with a p value <0.05. |
| Uitgever: |
Science publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 16 van 16 gevonden artikelen
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