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                                       Details for article 4 of 24 found articles
 
 
  Characterization of Bacterial Strains Isolated Through Microbial Profiling of Urine Samples
 
 
Title: Characterization of Bacterial Strains Isolated Through Microbial Profiling of Urine Samples
Author: Poulomi Nandy
A.R. Thakur
S. Ray Chaudhuri
Appeared in: OnLine journal of biological sciences
Paging: Volume 7 (2007) nr. 1 pages 44-51
Year: 2007
Contents: The present study was conducted to determine the microbial profile in urine samples. Differential and selective chromogenic culture media were used for the rapid detection, identification and enumeration of urinary tract pathogens namely, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Proteus mirabilis. Urine samples of normal healthy individuals as well as patients with Urinary Tract Infection (UTI) were screened onhicrome agar plates. The cultivable bacteria present in urine were isolated based on chromogenic detection. Antibiotic sensitivity assay, morphological characterization and biochemical tests, namelyprotease, oxidase, catalase, lipase, DNase and lecithinase assay were performed with the 15 isolates obtained from urine samples. The molecular analyses of the isolates were done through partial sequencing of the 16SrDNA gene; six of them were found to be novel and submitted in GenBank under the accession numbers EF644491-96. Phylogenetic tree of the isolates were constructed by neighbour joining method.
Publisher: Science publications (provided by DOAJ)
Source file: Elektronische Wetenschappelijke Tijdschriften
 
 

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