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| Titel: |
Callus induction studies in Tridax procumbens L. |
| Auteur: |
Minal Wani Snehal Pande Nitin More |
| Verschenen in: |
International journal of biotechnology applications |
| Paginering: | Jaargang 2 (2010) nr. 1 pagina's 11-14 |
| Jaar: |
2010 |
| Inhoud: |
Tridax procumbens is a common weed plant belonging to the family Asteraceae. The leaf juicehas antiseptic, insecticidal and parasiticidal properties. The present work is based on developing a protocolfor the callus induction in Tridax procumbens from various explants like leaf, internodes and shoot apicalbuds. The sterilized explants were inoculated in MS media containing various combination of auxins such as2, 4, dichlorophenoxy acetic acid (2, 4-D) and naphthalene acetic acid (NAA) and cytokinins such as kinetinand 6 benzyl amino purine (BAP). Leaf and apical bud explants showed early and profuse callus inductionwhereas internodal explants showed comparatively delayed but profuse callus induction. Leaf and apicalbud explants showed maximum response in terms of callus by using MS media with the combination 2, 4-D0.5mg/lit and BAP 0.5mg/lit which was followed by 2, 4-D 0.5mg/lit and KIN 0.5mg/lit, 2, 4 -D 0.5mg/lit andBAP+KIN 0.5 mg/lit, NAA 0.5mg/lit and BAP 0.5mg/lit, NAA 2mg/lit and BAP 0.5mg/lit respectively. Whereasinternodal explants showed maximum callus induction by using a hormonal concentration of 2mg/lit 2,4, Dand 0.5mg/lit BAP. In vitro generated callus can be used as a source for the isolation of secondarymetabolites from Tridax plant. |
| Uitgever: |
Bioinfo Publications (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
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