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Details van artikel 15 van 95 gevonden artikelen
| Titel: |
Callus Induction, Regeneration and Hygromycin Selection of Rice (Super Basmati) |
| Auteur: |
Hamid Rashid Syed Yassir Abbas Bokhari Azra Quraishi |
| Verschenen in: |
Journal of biological sciences |
| Paginering: | Jaargang 1 (2001) nr. 12 pagina's 1145-1146 |
| Jaar: |
2001 |
| Inhoud: |
A protocol has been developed for callus induction, regeneration and hygromycin selection of rice (Oryza sativa L. cv. Super Basmati). Mature seeds of rice were used as starting material for callus induction and cultured on Murashige & Skoog medium (MS) and N6 medium having different levels and combinations of 2,4-Dicholro Phenoxy Acetic Acid (2,4-D). 2,4-D @ 2 mg/l gave good response to callus induction. For regeneration, calli were cultured on MS medium having different concentrations of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP). Medium containing NAA @ 1mg/l with BAP 5 mg/l showed best results with regeneration frequency of 45.3%. Hygromycin at the concentration of 50 mg/l proved to be lethal for secutellum-darived calli of Super Basmati. |
| Uitgever: |
Asian Network for Scientific Information (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 15 van 95 gevonden artikelen
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