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Details for article 2 of 4 found articles
| Title: |
Heterochromatin density in the course of cell “dedifferentiation“ represented by blastic transformation of human mature T lymphocytes |
| Author: |
Karel Smetana Petra Otevrelova Ivan Kalousek |
| Appeared in: |
Journal of applied biomedicine |
| Paging: | Volume 5 (2007) nr. 4 pages 189-193 |
| Year: |
2007 |
| Contents: |
The present study was undertaken to provide more information on the heterochromatin density in centraland peripheral nuclear regions during “cell dedifferentiation“ represented by blastic transformation ofmature T lymphocytes. Heterochromatin was visualized using a simple cytochemical method for thedemonstration of DNA followed by computer-assisted densitometry of digitised images. The resultsindicated that the blastic transformation was accompanied by a marked and significant decrease in theheterochromatin density at the nuclear membrane. Thus, this nuclear peripheral region seems to beimportant not only for cell differentiation but also dedifferentiation events. It is also interesting that thenon-stimulated resting mature cells in the present study were characterized by less condensedheterochromatin at the nuclear membrane than differentiated granulocytic or erythrocytic precursors andapoptotic myeloblasts or leukemic B lymphocytes described in the previous study. However, in contrast tothese cells, resting and mature T lymphocytes in the present study are known to revert to cycling blasticcells after PHA treatment. In addition, it is also known that nuclear peripheral regions withheterochromatin represent sites of chromosomal attachments as well as “together crowded replicons“ andsilent genes. |
| Publisher: |
University of South Bohemia (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 2 of 4 found articles
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