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Details for article 19 of 21 found articles
| Title: |
Peroxidase from Leaves of Spinach (Spinacia oleracea): Partial Purification and Some Biochemical Properties |
| Author: |
E. Koksal |
| Appeared in: |
International journal of pharmacology |
| Paging: | Volume 7 (2011) nr. 1 pages 135-139 |
| Year: |
2011 |
| Contents: |
In present study, it is aimed to purify peroxidase (EC 1.11.1.7; donor: Hydrogen peroxide oxidoreductase) from leaves of spinach and determine its some biochemical properties. Peroxidase is an oxidoreductase enzyme produced by a number of organisms. Spinach (Spinacia oleracea L.) is an edible flowering plant in the family of Amaranthaceae and is heavily consumed all over the world. Peroxidase enzyme was purified from Leaves of Spinach (Spinacia oleracea L.) by ammonium sulphate precipitation and CM-Sephadex ion-exchange chromatography. Km (Michaelis constant) and Vmax (The maximum velocity of an enzymatic reaction) values were calculated from the Lineweaver-Burk graph for catechol and guaiacol substrates and the substrate specificity of the enzyme was investigated. The results have shown that guaiacol is a better substrate from catechol for this enzyme. Furthermore, for the guaiacol substrate, optimum pH, optimum temperature, optimum ionic strength, stable pH, conditions were determined. |
| Publisher: |
Asian Network for Scientific Information (provided by DOAJ) |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 19 of 21 found articles
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