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| Titel: |
Electrochemical DNA based biosensor for the detection of genotoxic compounds in fish bile Samples |
| Auteur: |
A.M. Tencaliec G. Bagni M. Mascini V. Magearu |
| Verschenen in: |
Analele Universitatii Bucuresti. Chimie |
| Paginering: | Jaargang XIV (2005) nr. I-II pagina's 179-186 |
| Jaar: |
2005 |
| Inhoud: |
A disposable electrochemical DNA based biosensor for the detection of genotoxiccompounds in fish bile samples as marker of PAHs (polycyclic aromatic hydrocarbons)exposured at contaminated sites has been reported. The DNA biosensor is assembled byimmobilising double stranded Calf Thymus DNA onto the surface of a disposable carbon screenprintedelectrode. The oxidation signal of the guanine base, obtained by a square wavevoltammetric scan, is used as analytical signal to detect the DNA damage; the presence of lowmolecular weight compounds with affinity for nucleic acids is measured by their effect on theguanine oxidation peak. Preliminary analysis of some PAH metabolites standard solutions wereperformed, in order to establish their behaviour with DNA based biosensor. Also, in order tohighlight the differences between the not exposed bile and the injected bile, a deconjugation (orhydrolisis) process of the bile has been performed.The applicability of such a biosensor foranalysing bile samples of fish from sites with high ecological risk was evaluated. |
| Uitgever: |
Ars Docendi Publishing House (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 50 van 143 gevonden artikelen
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