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Details van artikel 47 van 143 gevonden artikelen
| Titel: |
DNA-BASED BIOSENSOR FOR DETECTION OF GENETICALLY-MODIFIED ORGANISMS |
| Auteur: |
A.G. Nica M. Mascini A.A. Ciucu |
| Verschenen in: |
Analele Universitatii Bucuresti. Chimie |
| Paginering: | Jaargang XIII (2004) nr. I-II pagina's 85-94 |
| Jaar: |
2004 |
| Inhoud: |
An electrochemical genosensor for the monitoring of hybridization was used in orderto develop and characterize a DNA biosensor-based assay for the detection of geneticallymodifiedorganisms (GMOs). Screen-printed gold electrodes were modified with a suitable thioltetheredDNA sequence (probe) related to the sequence of the 35S promoter (target) inserted inthe genome of GMOs and responsible for regulating the transgene expression. An enzymeamplifieddetection scheme was applied in order to quantify the electrochemical signal producedby reaction between probe sequence and target sequence (analyte). The assay was firstlycharacterized using synthetic oligonucleotides. Relevant parameters such as probe concentration,hybridization and enzymatic reaction time were investigated and optimized. Electrochemicaltechniques for DNA-modified electrodes control were used. |
| Uitgever: |
Ars Docendi Publishing House (provided by DOAJ) |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 47 van 143 gevonden artikelen
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