Effet du milieu de culture sur le microbouturage de l'olivier (Olea europeae L.) cv. Picholine Marocaine
Titel:
Effet du milieu de culture sur le microbouturage de l'olivier (Olea europeae L.) cv. Picholine Marocaine
Auteur:
Brhadda N. Abousalim A. Walali Loudiyi D. E. Benali D.
Verschenen in:
Biotechnologie, agronomie, société et environnement
Paginering:
Jaargang 7 (2003) nr. 3-4 pagina's 177-182
Jaar:
2003
Inhoud:
Effect of culture medium on micropropagation of olive (Olea europeae L.) cv. Moroccan Picholine. The effect of the basal media OM (Olive Medium), 1/2 MS (Murashige et Skoog with half strength macronutrients), WPM (Lloyd and McCown), 1/2 Miller (Miller with half strength macronutrients), and K&H (medium with Knop macronutrients and Heller micronutrients), supplemented with 5 mg/l Zeatine, on shoot proliferation of mature Moroccan Picholine'cultivar (30 years old) was investigated. OM and 1/2 MS media were the most effective at the early stages of proliferation. A microcutting percentage of up to 91,6 and 90,9 % were achieved in OM and 1/2 MS media respectively but OM was distinguished later by permitting a better shoot growth with no vitrification symptoms The highest percentages of new shoots per explant were obtained with 1/2 MS and OM media (67 and 65 % respectively). OM was the most effective for shoot height (12,42 mm) followed by 1/2 MS (8,92 mm). The other tested media induced an important callus development and leaf chlorosis, and the reduction of shoot growth was noticeable.
Uitgever:
University of Gembloux, Faculty of Agricultural Sciences