Enzymatic Reduction of Methemoglobin to Hemoglobin in Blue Catfish Ictaburus furcatus, and Channel Catfish, I. punctatus, ♀ x Blue Catfish ♂ Hybrids
Titel:
Enzymatic Reduction of Methemoglobin to Hemoglobin in Blue Catfish Ictaburus furcatus, and Channel Catfish, I. punctatus, ♀ x Blue Catfish ♂ Hybrids
Auteur:
Reagan, Roland E. Drennan, Douglas G.
Verschenen in:
Journal of applied aquaculture
Paginering:
Jaargang 3 (1994) nr. 3-4 pagina's 223-234
Jaar:
1994-06-01
Inhoud:
Blue catfish, "Ictalurus furcatus," and channel catfish, "I. punctatus," (strain unknown) ♀ X Rio Grande strain blue catfish ♂ hybrids (HRG), and channel catfish (the same unknown strain) ♀ X Tombigbee blue catfish ♂ (HTB) were exposed to 0, 5, 10, and 20 mg/L nitrite to determine percent methemoglobin and enzymatic activity of NADH-methemoglobin reductase. Channel catfish of an unknown strain were exposed to 0 and 20 mg/L nitrite and compared to blue catfish, HRG catfish, and HTB catfish. Percent methemoglobin increased with increasing nitrite concentration for all groups of fish. Percent methemoglobin at 5 mg/L nitrite in blue catfish was significantly lower (P < 0.05) than those of HRG catfish or HTB catfish. Channel catfish had significantly (P < 0.05) higher NADH-methemoglobin reductase than blue catfish, HRG catfish, and HTB catfish. At all the levels of nitrite, there was no significant difference between blue catfish, or HTB catfish for NADH=MH-b reductase.
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