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Details van artikel 11 van 23 gevonden artikelen
| Titel: |
INDUCTION OF 8-HYDROXY-2'-DEOXYGUANOSINE BY COBALT(II) AND HYDROGEN PEROXIDE IN VITRO |
| Auteur: |
Ivancsits, Sabine Diem, Elisabeth Pilger, Alexander Rudiger, Hugo W. |
| Verschenen in: |
Journal of toxicology and environmental health. Part A |
| Paginering: | Jaargang 65 (2002) nr. 9 pagina's 665-676 |
| Jaar: |
2002-05-10 |
| Inhoud: |
In order to test the effects of cobalt on oxidative DNA damage, we measured the formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the presence of cobalt in calf thymus DNA and in DNA of human diploid fibroblasts. Treatment of calf thymus DNA with Co(II) at 500 µ M hydrogen peroxide resulted in a dose-dependent increase in 8-OHdG, which culminated at 25 µ M Co(II) (62.6 modified/105 dG) and declined at higher Co(II) concentrations [9.6 modified/10 5 dG at 250 µ M Co(II)]. Incubation of calf thymus DNA with Co(II) alone did not cause an increase in 8-OHdG. Treatment of calf thymus DNA with H 2 O 2 (0.25-2 m M ) caused only a slight generation of 8-OHdG (2.7/10 5 dG, at 2 m M H 2 O 2 ). Exposure of human diploid fibroblasts to Co(II) at 5-250 µ M did not result in an increase in 8-OHdG in a dose-dependent manner, although treated cells showed significantly higher 8-OHdG levels than untreated controls (2.026 ±0.695 vs. 1.395 ±0.433 8-OHdG/10 5 dG) at all concentrations. Our data indicate that in the presence of H 2 O 2 , Co(II) stimulates the in vitro formation of 8-OHdG. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 11 van 23 gevonden artikelen
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