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Details van artikel 14 van 138 gevonden artikelen
| Titel: |
An Improved Method for the Synthesis of Gdp-Hexanolamine Derivatives, key Reagents for the Purification and Characterisation of Carbohydrate Processing Enzymes |
| Auteur: |
Bamford, Mark Britten, Christopher Draeger, Eleanor Gore, Paul Holmes, Duncan S. |
| Verschenen in: |
Journal of carbohydrate chemistry |
| Paginering: | Jaargang 15 (1996) nr. 6 pagina's 727-737 |
| Jaar: |
1996-08-01 |
| Inhoud: |
As part of our ongoing studies on the isolation and characterisation of α-(1,3)-fucosyltransferase (FucT) enzymes, an improved method for the synthesis and purification of the key biological tool GDP-hexanolamine (1a), together with detailed experimental conditions and product characterisation are reported. We demonstrate the viability of the product obtained, by its competitive inhibition of an α-(1,3)-fucosyltransferase. Subsequently, we are able to use it to derivatise a sepharose gel column which we show is highly efficient in the purification of a recombinant α-(1,3)-fucosyltransferase from a crude preparation. The GDP-hexanolamine is converted to the photoaffinity probe GDP-hexanolaminyl-4-azidosalicylate (1b), useful in the characterisation of fucosyltransferase enzymes. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 14 van 138 gevonden artikelen
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