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| Titel: |
Effects of asimilobine on dopamine biosynthesis and l-DOPA-induced cytotoxicity in PC12 cells |
| Auteur: |
Jin, Chun-Mei Lee, Jae-Joon Kim, Young-Kyoon Ryu, Shi-Yong Lim, Sung-Cil Hwang, Bang-Yeon Lee, Chong-Kil Lee, Myung-Koo |
| Verschenen in: |
Journal of Asian natural products research |
| Paginering: | Jaargang 10 (2008) nr. 8 pagina's 737-745 |
| Jaar: |
2008-08 |
| Inhoud: |
The effects of asimilobine, an aporphine isoquinoline alkaloid, on dopamine biosynthesis and l-DOPA-induced cytotoxicity in PC12 cells were investigated. Asimilobine at concentration ranges of 0.05-0.2 μM showed a significant inhibition of intracellular dopamine levels for 24 h in a concentration-dependent manner with an IC50 value of 0.13 μM. Asimilobine at 0.15 μM inhibited tyrosine hydroxylase (TH) and aromatic l-amino acid decarboxylase (AADC) activities at 24 h (73.2% inhibition of TH activity): the inhibition of TH activity was stronger and longer than that of AADC activity. Asimilobine also decreased TH mRNA levels and intracellular cyclic AMP levels, but not the basal Ca2 + concentrations. In addition, asimilobine at 0.05-5.0 μM, but not 10 μM, did not alter cell viability toward PC12 cells. A non-cytotoxic asimilobine (0.15 μM) associated with l-DOPA (20, 50, and 100 μM) for 24 h inhibited l-DOPA-induced increases in dopamine levels and enhanced l-DOPA-induced cell death when compared with l-DOPA alone. These results suggest that asimilobine inhibits dopamine biosynthesis by mainly reducing the TH activity and TH mRNA expression, and enhances l-DOPA-induced cytotoxicity in PC12 cells. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 12 van 43 gevonden artikelen
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