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Details van artikel 6 van 29 gevonden artikelen
| Titel: |
Elevated levels of DNA repair enzymes and antioxidative enzymes by ( )-catechin in murine microglia cells after oxidative stress |
| Auteur: |
Huang, Qing Wu, Li-Jun Tashiro, Shin-Ichi Onodera, Satoshi Ikejima, Takashi |
| Verschenen in: |
Journal of Asian natural products research |
| Paginering: | Jaargang 8 (2006) nr. 1-2 pagina's 61-71 |
| Jaar: |
2006-01 |
| Inhoud: |
(+)-Catechin possesses a broad range of pharmacological properties, including antioxidative effect. However, little is reported on the mechanism by which (+)-catechin protects microglia cells from DNA damage by oxidative stress. In this study, TUNEL assay and DNA electrophorysis indicated that (+)-catechin markedly blocked DNA fragmentation and apoptosis of microglia cells by tBHP exposure. A potent antioxidative effect of (+)-catechin was confirmed by comparison with a putative antioxidant agent, N-acetylcysteine at the lower doses. Furthermore, the increased intracellular ROS by tBHP exposure were scavenged by elevated activities of catalase (CAT) and superoxide dismutase (SOD) after (+)-catechin treament. (+)-Catechin partially inhibited the activation of caspase-3, thereby both cleavage of poly (ADP-ribose) polymerase (PARP) and degradation of inhibitor of caspase-activated DNase (ICAD) were effectively abolished. In addition, the expression of PARP for repair of impaired DNA was significantly increased by (+)-catechin treatment. Taken together, these data suggest that protective effects of (+)-catechin against oxidative DNA damage of microglia cells is exerted by the increased expression of DNA repair enzyme PARP and antioxidant enzyme activities. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 6 van 29 gevonden artikelen
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