|
| << vorige | volgende >> |
Tijdschrift beschrijving
Alle jaargangen van het bijbehorende tijdschrift
Alle afleveringen van het bijbehorende jaargang
Alle artikelen van de bijbehorende aflevering
Details van artikel 10 van 28 gevonden artikelen
| Titel: |
Determination of phosphate in freshwaters by flow injection with immobilized enzyme and chemiluminescence detection |
| Auteur: |
Yaqoob, Mohammad Anwar, Mohammad Nabi, Abdul |
| Verschenen in: |
International journal of environmental analytical chemistry |
| Paginering: | Jaargang 85 (2005) nr. 7 pagina's 451-459 |
| Jaar: |
2005-06-15 |
| Inhoud: |
A flow injection method for the determination of phosphate in freshwaters is described using immobilized pyruvate oxidase based on luminol chemiluminescence (CL) detection. Hydrogen peroxide released by the enzymatic reaction of pyruvate in the presence of phosphate is coupled with luminol in an alkaline medium generated CL response using cobalt(II) as a catalyst. The calibration graph was linear over the range of 2-10 × 10-6 M phosphate (r2 = 0.9985) with relative standard deviation (n = 5) 1.8-3.8%. The detection limit was 2 × 10-7 M with a sample throughput of 60 h-1. The common interferences were removed off-line by treating water samples with iminodiacetate chelating resin. The method was applied to freshwater samples and the results obtained [1.05-1.58 × 10-6 M (0.10 ± 0.02-0.15 ± 0.01 mg [image omitted] L-1)] were in reasonable agreement with the results obtained using the molybdenum blue method with spectrophotometric detection [1.47-1.89 × 10-6 M (0.14 ± 0.03-0.18 ± 0.02 mg [image omitted] L-1)]. The immobilized pyruvate oxidase exhibited good operational activity at 30°C for a week and storage stability at 4°C over a period of three months. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 10 van 28 gevonden artikelen
| << vorige | volgende >> |