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| Titel: |
UTILIZATION OF PRETREATED MOLASSES FOR SERINE ALKALINE PROTEASE PRODUCTION WITH RECOMBINANT BACILLUS SPECIES |
| Auteur: |
Calik, Guzide Pehlivan, Nur Kalender, Nermi Ozdamar, Tuncer H. Calik, Pinar |
| Verschenen in: |
Chemical engineering communications |
| Paginering: | Jaargang 190 (2003) nr. 5-8 pagina's 630-644 |
| Jaar: |
2003-05-01 |
| Inhoud: |
Recombinant Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis , and Bacillus licheniformis were used for the production of serine alkaline protease (SAP) utilizing chemically and/or physically pretreated molasses. The highest enzyme activity was obtained with r- Bacillus subtilis , with the complex medium involving physically treated molasses having 20 kg m -3 initial sucrose concentration in small-scale, agitation- and heating rate-controlled bioreactors at t=63 h. Effects of oxygen transfer were investigated in 3.5 dm 3 laboratory bioreactors under two different agitation rates with r- B. subtilis . Increase in the oxygen transfer rate increased the observed activity and caused the cultivation time of maximum activity shift to the earlier stages of the fermentation. At Q/V=0.5 vvm and N=750 min -1 , SAP activity reached 2250U cm -3 at t=36 h. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 36 van 36 gevonden artikelen
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