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Details for article 5 of 8 found articles
| Title: |
METHOD FOR NON-INVASIVE CONCENTRATION MEASUREMENT IN POLYMER MIXTURES: TESTS WITH DEXTRAN AND CYTOCHROME-C |
| Author: |
Zhang, Wei Ethier, C. Ross |
| Appeared in: |
Chemical engineering communications |
| Paging: | Volume 189 (2002) nr. 1 pages 101-113 |
| Year: |
2002-01 |
| Contents: |
Non-invasive measurement of solute concentrations in a binary macromolecule system is a powerful tool for the study of mass transport phenomena, particularly for studying solute-solute interactions. Our objective is to extend an existing differential refractometric technique for measurement of concentrations in a two-macromolecule system. Methods We used a specially designed cell and associated optical equipment to measure the local refractive index and light extension coeffcient of a polymer mixture. The macromolecules used were dextran (molecular weight 2 million) and the protein cytochrome-c. A three-layered calibration procedure quantified the refraction and light extinction due to each of these components in a mixture, as well as the interaction between the two components. Results Calibration data showed that dextran and cytochrome-c concentrations could be determined from mixture properties with good accuracy and repeatability. Specifically, for cytochrome-c over the concentration range from 0.1-0.6 mass, the maximum uncertainty in measured concentration was approximately 0.013%, expressed as a 95% confidence interval for the measurement. The corresponding maximum uncertainty for dextran over the concentration range from 0.1-0.6 mass% was 0.037%. Conclusion The experimental approach proposed herein is suitable for the measurement of transport in binary polymer mixtures where one or both components are fairly strongly light-absorbing. It allows real-time monitoring of macromolecular transport without radioactive tracers or other complex equipment. |
| Publisher: |
Taylor & Francis |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 5 of 8 found articles
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