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| Titel: |
Effect of Light Quality on the Circadian Expression of Nitrate Reductase in the Red Macroalga Gracilaria tenuistipitata |
| Auteur: |
Lopes, Patricia Fatima Santa-Maria, Ulisses Rafael Colepicolo, Pio |
| Verschenen in: |
Biological rhythm research |
| Paginering: | Jaargang 33 (2002) nr. 4 pagina's 391-400 |
| Jaar: |
2002-10 |
| Inhoud: |
A circadian rhythm in the activity of nitrate reductase (NR: EC 1.6.6.1) isolated from the marine red algae Gracilaria tenuistipitata is shown to be attributable to the daily oscillation of protein levels. The experiments reported here indicate that light quality has differential effects over NR expression. In extracts of algae grown under white light : dark, red light : dark and blue light : dark cycle, the activity of NR peaks during photophase, as does photosynthesis. Staining with a monoclonal antibody (NR10), raised against NR purified from Porphyra yezoensis, shows that the amount of protein changes by a factor of about 20, with a maximum occurring during photophase when algae are submitted to white and blue light. Red light changes the circadian rhythm of NR protein levels and also inhibits its night degradation. Illumination with blue light is able to restore the NR activity as well as its protein levels only when the light irradiance was the same of the white light. Surprisingly, the red light promoted 40% induction on NR activity under the same conditions. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 9 gevonden artikelen
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