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| Titel: |
Modulation of cell cycle phases by various circadian and noncircadian light/dark schedules and evidence implicating melatonin |
| Auteur: |
Wright, Mary L. Jorey, Suzanne T. Blanchard, Linda S. Garatti, Linda Mayrand, Shawn M. Kraszewska, Anna A. |
| Verschenen in: |
Biological rhythm research |
| Paginering: | Jaargang 24 (1993) nr. 2 pagina's 101-117 |
| Jaar: |
1993-05 |
| Inhoud: |
The influence of circadian (6L:18D, 18L:6D) and noncircadian (12L:18D, 15L:15D) light/dark (LD) schedules on the cell cycle was studied in the hindlimb epidermis of late premetamorphic tadpoles of the frog, Rana pipiens. Using tritiated thymidine autoradiography, percent labeled mitoses (PLM) curves were obtained for 3 cell cycle determinations on each LD schedule, starting at 0, 8, and 16 hr for the 24 hr, and at 0, 10, and 20 hr for the 30 hr LD regimens. From the PLM curves, the durations of the whole cycle (T), the DNA synthetic phase (S), and the pre- (G1+1/2M) and post- (G2+1/2M) synthetic gaps, which included mitotic time, were determined. The data were compared with previous work on 12L:12D. The shape of the PLM curves and the resulting phase durations were characteristic for each LD regimen. T was longer where L was less than D and shorter where L was greater than D. The relative duration of S decreased, while G1+1/2M increased, as L lengthened on the 24 hr regimens, whereas on the 30 hr regimens the opposite occurred. On both 24 and 30 hr schedules, as L lengthened the percentage of cell cycle time in G2+1/2M increased. The findings show the specific influence of a change in the LD schedule, including noncircadian regimens, on particular cell cycle phases in a vertebrate. In a separate experiment, the labeling index was studied over a 24 hr period in control tadpoles and in those treated with hydrocortisone or melatonin. Since only melatonin shifted the phase of the labeling index rhythm when injected during the light, this hormone may account for the effects of the LD schedule on the cell cycle. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 5 van 8 gevonden artikelen
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