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Details for article 7 of 11 found articles
| Title: |
Saliva-mediated inhibition and promotion of bacterial adhesion to polystyrene |
| Author: |
Eli, Ilana Judes, Herbert Rosenberg, Mel |
| Appeared in: |
Biofouling |
| Paging: | Volume 1 (1989) nr. 3 pages 203-211 |
| Year: |
1989-03 |
| Contents: |
A simple model system was developed in order to study the effect of saliva on bacterial adhesion to a hydrophobic model surface, polystyrene. Washed bacterial suspensions of Acinetobacter calcoaceticus RAG-1 (ATCC 31012) and Serratia marcescens RZ were mixed with serial dilutions of clarified saliva, and the mixtures incubated in wells of untreated polystyrene microtiter plates. Adhesion was recorded spectro-photometrically after staining of adherent cells. Saliva inhibited adhesion of S. marcescens RZ in a concentration-dependent manner. Of interest was the finding that saliva exerts two distinct effects on adhesion of A. calcoaceticus RAG-1. It inhibits adhesion at low saliva concentrations, but causes aggregation of RAG-1 cells and stimulation of adhesion at high saliva concentrations. Extraction of saliva with hexadecane. prior to the adhesion assay, brought about an almost complete loss of the saliva-mediated inhibition of adhesion in both strains. This same treatment, however, did not prevent the aggregation of RAG-1 cells and the concomitant promotion of adhesion at high saliva concentrations. Initial evidence suggests that the components responsible for saliva-mediated inhibition of adhesion to polystyrene are of low ( < 15 kD) molecular weight. |
| Publisher: |
Taylor & Francis |
| Source file: |
Elektronische Wetenschappelijke Tijdschriften |
Details for article 7 of 11 found articles
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