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Details van artikel 10 van 15 gevonden artikelen
| Titel: | |
| Auteur: | |
| Verschenen in: |
Analytical letters |
| Paginering: | Jaargang 32 (1999) nr. 7 pagina's 1301-1312 |
| Jaar: |
1999 |
| Inhoud: |
Simultaneous determination of D- and L-lactic acids was achieved using an apparatus containing two enzyme reactors and an octadecylsilica column placed between the two reactors in tandem in a single flow line. The enzymes used for the D-lactic acid assay were D-lactate dehydrogenase and glutamic-pyruvic transaminase, and for the L-lactic acid assay, L-lactate dehydrogenase and glutamic-pyruvic transaminase. The optimal concentrations of L-glutamic acid and NAD+ in the carrier were determined. Of the buffers examined for use as a carrier medium, Tris buffer was found to be the most favorable. Under these optimal conditions, NADH due to D-lactic acid and that due to L-lactic acid could be observed separately in this apparatus. The calibration curve for D-lactic acid was linear in the range of 20 - 1000 μM, and that for L-lactic acid was 2 - 250 μM. This method was applied to analyze D- and L-lactic acids in several beverages that contained lactic acid bacteria. The D- and L-lactic acid content determined by the present method agreed with that determined by a commercially available test-kit method. |
| Uitgever: |
Taylor & Francis |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 10 van 15 gevonden artikelen
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