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| Titel: |
Activation of A3 receptors by endogenous adenosine inhibits synaptic transmission during hypoxia in rat cortical neurons |
| Auteur: |
Hentschel, S. Lewerenz, A. Nieber, K. |
| Verschenen in: |
Restorative neurology and neuroscience |
| Paginering: | Jaargang 21 (2003) nr. 1-2 pagina's 55-63 |
| Jaar: |
2003-07-11 |
| Inhoud: |
Purpose: The aim of our study was to characterize the influence of A3 receptors on synaptic potentials (PSPs) in pyramidal cells from the rat cingulate cortex during hypoxia. METHODS: Intracellular recordings (n = 49) were taken from slice preparations. PSPs were evoked by electrical stimulation. Results: Hypoxia (95%N2-5%CO2, 5 min) reduced the amplitude of the PSPs significantly. The effect was more pronounced in the presence of adenosine re-uptake inhibitor S-(p-nitrobenzyl)-6-thioguanosine (NBTG) and deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl)adenine (EHNA); the effect was completely reversed by bovine adenosine deaminase. Hypoxic inhibition induced after A1 receptor blockade with 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) in the presence of NBTG was completely reversed by the A3 antagonist 9-chloro-2-(2-furanyl)-5-[(phenylacetyl)amino]-1,2,4-triazolo[1,5-c]quinazoline (MRS 1220), indicating the involvement of A3 receptors in hypoxic PSP inhibition. This was confirmed by A3 agonist N6-(3-iodobenzyl)-adenosine-5′-N-methylcarboxamide (IB-MECA) inhibiting PSPs. The effect of IB-MECA was blocked by the rat A3 receptor-selective antagonist 3-propyl-6-ethyl-5-[(ethylthio)carbonyl]-2-phenyl-4-propyl-3-pyridinecarboxylate (MRS 1523) and was not observed in the presence of G-protein inhibitor guanosine-5′-O-2-thiodiphosphate (GDP-β-S). Conclusion: We conclude that a high level of endogenous adenosine, which occurs during hypoxia, activates A3 receptors. Their activation contributes to PSP inhibition by adenosine during hypoxia. |
| Uitgever: |
IOS Press |
| Bronbestand: |
Elektronische Wetenschappelijke Tijdschriften |
Details van artikel 4 van 37 gevonden artikelen
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